Want to find out whether the Mobile HomeCage fits into your experimental set-up? In this guide to compatibility, we provide the instructions for taking the microscope measurements, selecting a stage, and an air source. If you don’t want to measure the microscope yourself, tell us the maker and the model. Ideally, also send us a front and side picture of your setup with a ruler.
If you are measuring the microscope yourself, watch the video below and read the instructions on taking the measurements.
The Mobile HomeCage models
Let’s recapitulate some basic facts about the Mobile HomeCage. It comes in two sizes: the standard size Mobile HomeCage is compact and compatible with the majority of commercial 2-photon microscopes. The Mobile HomeCage Large allows combining imaging with behavioral readouts. For this reason, the floating cage has a larger diameter (325 mm compared to 180 mm in the standard-size Mobile HomeCage). The frame is also larger: it requires more space in the imaging setup. Both systems can be equipped with locomotion tracking components. Luckily, the sensor board used for tracking is built into the air dispenser. It does not require additional space in the setup. Whether you are using the system with or without locomotion tracking, it takes up the same space.
Measuring the microscope for compatibility
The picture on the left is a symbolic representation of a microscope frame.
Letters A through F stand for critical dimensions. Pay special attention to the first three measurements.
Compatibility table
The table below explains what the critical dimensions are, what their values must be, and what to do if your measurements do not match the given values. The values are given for the default configuration with the standard rotating clamp:
| Dimensions | Mobile HomeCage | Mobile HomeCage Large | Possible fixes |
|---|---|---|---|
| A. Distance from the objective's focal point to the microscope frame must be | >122 mm | >272 mm | Mobile HomeCage: use an elongated clamp (min distance with elongated clamp 102 mm); Mobile HomeCage Large: use a smaller cage diameter (ask us about customization) |
| B. Distance from the objective's focal point (objective retracted) to the table / stage surface must be | >70 mm | >84 mm | Lower the stage (to increase the distance); lift the stage or use a riser block (to decrease the distance) |
| C. Objective's parfocal length must be | >40 mm | >40 mm | Use a parfocal length extender to increase the objective's length or use a low-wall cage with a short neck |
| D. Objective's length with holder / slider must be | >56 mm | >56 mm | Use a short neck and a low-wall cage |
| E. Objective's diameter | Must be considered in conjunction with the working distance - contact us | Under ceratain conditions and with certain changes to the standard configuration, it is possible to use up to 100 mm diameter objectives. | |
| F. Objective's holder diameter must be | <80 mm | <80 mm | Use an elongated clamp |
The Mobile HomeCage® Large is compatible with these commercial two-photon microscopes:
- without modifications: Scientifica’s HyperScope (VivoScope frame with TOM), Neurolabware, Nikon’s A1R and AX R (on the gate stands), Sutter MOM, Femtonics’ FemtoSmart (with the Bridge structure), Olympus FVMPE-RS (gantry frame with InnerFocus); Thorlabs’ Bergamo II (recent development, info may not have spread internally but we can connect you to the right people); Bruker’s (resurrected) Ultima 2P Plus Extended Reach model is also fully compatible (we use it at Neurotar) – unfortunately there is no info available on the web about the Extended Reach frame – ask Bruker directly or ask our team who to contact at Bruker;
- with some modifications: Bruker’s Ultima Investigator and 2P Plus models (smaller diameter cages), Femtonics’ Femto3D Atlas (parfocal objective length extension, several standard parts’ replacement), Thorlabs’ DIY 2-photon (smaller diameter cages);
- Finally, it is possible to use the Mobile HomeCage Large system with most commercial microscopes by adding a periscopic extension, such as the InverterScope available from LSM Tech.
Selecting a stage
Make sure that the stage can carry the weight of your Mobile HomeCage device. The stage surface can be slightly smaller than the size of the air dispenser. Check the table below for the unpackaged weights and air dispenser sizes (length X width) of the Mobile HomeCage systems:
| Type of device | Unpackaged weight (kg) | Air dispenser dimensions (mm) |
|---|---|---|
| Mobile HomeCage | 6.1 | 420 X 300 |
| Mobile HomeCage with tracking capability | 6.8 | 420 X 300 |
| Mobile HomeCage Large | 11.5 | 720 X 570 |
| Mobile HomeCage Large with tracking capability | 13 | 720 X 570 |
| MultiCage Training Arena | 11.5 | 720 X 570 |
Sample stages for the Mobile HomeCage systems
- Prior motorized XY stepper stage (carries up to 100 kg; top plate 300 mm X 300 mm). Contact us for an offer.
- Prior heady-duty translation stage (carries up to 100 kg, top plate 600 mm X 600 mm, low vertical profile) – this stage is optimal for integrating the Mobile HomeCage Large into imaging setups equipped with periscopic objective extensions such as the InnerFocus (Evident Scientific, formerly Olympus) or InverterScope (LSM-Tech). HT11300KT-NL (not yet shown on the Prior website – contact us for more information).
- Prior ZDeck: motorized or manual (carries up to 25 kg)
- ThorLabs’ PMP-2XY/M translational stage (motorized stage carries 34 kg)
- Scientifica’s Motorised Movable Base Plate (MMBP) (stage carries 35 kg)
Use four fixation slots (compatible with metric and imperial breadboards) to secure the Mobile HomeCage to the stage. Alternatively, use Neurotar’s fixation kit (not included with the Mobile HomeCage system).
The surface of the air dispenser of the Mobile HomeCage Large exceeds the surface of most stages (see the picture on the left: Mobile HomeCage Large on Thorlabs’ stage). Secure the air dispenser to the stage using two out of four fixation slots.
If you frequently move the standard-size Mobile HomeCage in and out of the imaging setup, we recommend using the fixation kit for quickly fixing and securing the Mobile HomeCage in the exact same spot on the XY stage.
Selecting an air source
To operate your Mobile HomeCage device effectively, you’ll need a high-flow, low-pressure air source. The table below outlines airflow requirements for each Mobile HomeCage system type:
| Type of device | Air source specifications |
|---|---|
| Mobile HomeCage | 120 L/min at 20 kPa |
| Mobile HomeCage with tracking capability | 120 L/min at 20 kPa |
| Mobile HomeCage Large | 150 L/min at 20 kPa |
| Mobile HomeCage Large with tracking capability | 150 L/min at 20 kPa |
| MultiCage Training Arena | 250 L/min at 20 kPa |
Most labs use their existing lab air lines, though some require an external portable air source. Any pump or blower that meets or exceeds these specifications will work. Note: We don’t recommend using compressed air tanks due to high pressure and rapid depletion rates, which make them impractical.
Recommended portable air sources
Industrial air blowers: Ideal for continuous, high-flow performance without overheating. These often have adjustable airflow, though they can be costly and may require installation by a certified electrician. Neurotar offers a plug-and-play solution based on Leister’s Mono-6 industrial air pump, which we highly recommend. Learn more and order online in our webshop.
Aquarium pumps: Some cost-effective aquarium pumps can work well, and many are readily available on Amazon. However, most lack adjustable airflow. In these cases, we suggest purchasing an airflow regulator. Some aquarium pumps may cause unwanted vibrations; if this happens, we have a quick fix. Watch this video for more details, or contact us for troubleshooting tips and specific recommendations, as options may vary by location.
Video note: The industrial air pump featured in this video is the Leister Robust. In 2024, we updated our recommendation to Leister’s Mono-6 due to its compact design, integrated airflow adjustment, and cost-efficiency. We will update the video soon.
Using your existing air source with MultiCage
Is your current air source for the Mobile HomeCage or Mobile HomeCage Large incompatible with the MultiCage? There’s a simple, low-cost solution to use the MultiCage at airflow levels below 250 L/min. Download the DIY instructions here:
Air pressure regulation
For stable air pressure, we recommend adding a basic air pressure regulator to your airline. A simple model is usually sufficient, but models with moisture and debris filters offer added benefits and are widely available on Amazon. Note: Avoid using a pressure regulator with aquarium pumps, as it can increase resistance, potentially requiring additional airflow and increasing noise.
Noise reduction tips
Most noise in the system comes from the air source and connectors. To reduce noise, try increasing the distance between the Mobile HomeCage and the air source using a longer hose and increasing airflow. Alternatively, consider enclosing the air source in a box for sound insulation.
