Mobile HomeCage References

More than 220 labs worldwide use the Mobile HomeCage devices for research in awake head-fixed mice. On this page, we have compiled the Mobile HomeCage references. They include publications, links to educational webinars, video testimonials, and a list of research institutions that use our equipment. Use the menu below to navigate to a specific category:


Kim, M.G, Yu, K., Yeh, CY., Fouda, R., Argueta, DA., Kiven, SB., Ni, Y., Niu, X., Chen, Q., Kim, K., Gupta, K., He, B. (Blood, 2024) Low-intensity transcranial focused ultrasound suppresses pain by modulating pain processing brain circuits.

Chini, M., Hnida, M., Kostka, J., Chen, Y., Hanganu-Opatz, I. L. (Cell Reports, 2024) Preconfigured architecture of the developing mouse brain.

Stuart, S.A., Palacios-Filardo, J., Domanski, A., Udakis M., Jones M. W., Mellor J. R. (Sci Rep, 2024) Hippocampal-dependent navigation in head-fixed mice using a floating real-world environment.

Bandet, M. V., Winship, I. R. (eLife, 2024) Aberrant cortical activity, functional connectivity, and neural assembly architecture after photothrombotic stroke in mice.

Bosch, P.J., Kerr, G., Cole, R., Warwick, C., Wendt, L., Pradeep, A., Barnall, E., Aldridge, G. (Cell Mol Neurobiol, 2024). Enhanced Spine Stability and Survival Lead to Increases in Dendritic Spine Density as an Early Response to Local Alpha-Synuclein Overexpression in Mouse Prefrontal Cortex.

Liebergall, S., & Goldberg, E. M. (Journal of Neuroscience, 2024). Ndnf interneuron excitability is spared in a mouse model of Dravet syndrome.

Rozov, S., Saarreharju, R., Khirug, S., Storvik, M., Rivera, C., & Rantamäki, T. (European Journal of Pharmacology, 2024). Effects of nitrous oxide and ketamine on electrophysiological and molecular responses in the prefrontal cortex of mice: A comparative study.

Pöpplau, J. A., Schwarze, T., Dorofeikova, M., Pochinok, I., Günther, A., Marquardt, A., & Hanganu-Opatz, I. L. (Neuron, 2024). Reorganization of adolescent prefrontal cortex circuitry is required for mouse cognitive maturation.

Fok AHK, Huang Y, So BWL, Zheng Q, Tse CSC, Li X, Wong KK, Huang J, Lai KO, Lai CSW. (Cell Rep. 2024). KIF5B plays important roles in dendritic spine plasticity and dendritic localization of PSD95 and FMRP in the mouse cortex in vivo.

Haruwaka, K., Ying, Y., Liang, Y., Umpierre, A. D., Yi, M. H., Kremen, V., … & Wu, L. J.  (Nat Neurosci, 2024). Microglia enhance post-anesthesia neuronal activity by shielding inhibitory synapses.

Stern, M. A., Cole, E. R., Gross, R. E., Berglund, K. (Neurophotonics, 2024) Seizure event detection using intravital two-photon calcium imaging data.

Fiore, F., Alhalaseh, K., Dereddi, R.R., Torres.F., Coban, I., Harb, A, Agarwal, A. (Nat Commun, 2023.) Norepinephrine regulates calcium signals and fate of oligodendrocyte precursor cells in the mouse cerebral cortex.

Bertolo, A., Ferrier, J., Cazzanelli, S., Diebolt, S., Tanter, M., Pezet, S., Pernot, M., Osmanski, B. & Deffieux, T. (Imaging Neuroscience, 2023) High sensitivity mapping of brain-wide functional networks in awake mice using simultaneous multi-slice fUS imaging.

Shimoda, Y., Leite, M., Graham, R.T., Marvin, J.S., Hasseman, J., Kolb, I., Looger, L.L., Magloire, V., Kullmann, D.M. (Brain, 2023) Extracellular glutamate and GABA transients at the transition from interictal spiking to seizures.

Goff, K. M., Liebergall, S. R., Jiang, E., Somarowthu, A., Goldberg, E. M. (Cell Reports, 2023) VIP interneuron impairment promotes in vivo circuit dysfunction and autism-related behaviors in Dravet syndrome

Case, S. L., Lin, R.-L., Thibault, O. (Aging Cell, 2023) Age- and sex-dependent alterations in primary somatosensory cortex neuronal calcium network dynamics during locomotion.

Luo, P., Yang, F., Li, J., Niemeyer, J.E., Zhan, F., Estin, J., Zhao, M., Li, D., Lin, W., Liou, J.-Y., Ma, H., Schwartz, T.H. (Epilepsia, 2023). Excitatory–inhibitory mismatch shapes node recruitment in an epileptic network.

Smith, J.A., Ji, Y., Lorsung, R., Breault, S. M., Koenig, J., Cramer, N., Masri, R., Keller, A. (Journal of Neuroscience, 2023) Parabrachial nucleus activity in nociception and pain in awake mice

Tessier, M., Garcia, S. M., Goubert, E.,  Blasco, E., Consumi, A., Dehapiot, B., Tian, Li., Molinari, F.,  Laurin, J., Guillemot, F., Hübner, C., Pellegrino, C., Rivera, C. (Brain, 2023). Bumetanide induces post-traumatic microglia-interneuron contact to promote neurogenesis and recovery.

Fedotova, A.,  Brazhe, A., Doronin, M., Toptunov, D., Pryazhnikov, E.,  Khiroug, L., Verkhratsky, A., Semyanov, A. (Function, 2023). Dissociation Between Neuronal And Astrocytic Calcium Activity in Response to Locomotion in Mice

Sit, K., Goard, M. (Nature Communications, 2023). Coregistration of heading to visual cues in retrosplenial cortex.

Krogsgaard, A., Sperling, L., Dahlqvist, M., Thomsen, K., Vydmantaite, G., Li, F., Thunemann, M., Lauritzen, M., & Lind, B. L. (Glia, 2023). PV interneurons evoke astrocytic Ca2+ responses in awake mice, which contributes to neurovascular coupling.

Parrish R. R., Mackenzie-Gray-Scott, C., Jackson-Taylor, T., Grundmann, A., McLeod, F., Codadu, N.K., Călin, A., Alfonsa, H., Wykes, R. C., Voipio, J. & Trevelyan, A. J. (Journal of Neuroscience, 2022). Indirect effects of Halorhodopsin activation: potassium redistribution, non-specific inhibition and spreading depolarization.

Zong, F. J., Min, X., Zhang, Y., Li, Y. K., Zhang, X. T., Liu, Y., & He, K. W. (The EMBO Journal, 2022). Circadian time- and sleep-dependent modulation of cortical parvalbumin-positive inhibitory neurons.

Gómez L. J., Dooley J. C., Blumberg, M. S. (eLife, 2022) Activity in the developing prefrontal cortex is shaped by sleep and sensory experience.

Jafarian, A., Wykes, R. C. (Front. Comput. Neurosci., 2022). Impact of DC-coupled electrophysiological recordings for translational neuroscience: case study of tracking neural dynamics in rodent models of seizures.

Redman et al. (eLife, 2022). Long-term transverse imaging of the hippocampus with glass micro-periscopes

Kucharz et al. (Pharmaceutical Research, 2022). Shedding Light on the Blood–Brain Barrier Transport with Two-Photon Microscopy In Vivo.

Karalis, N., Sirota, A. (Nature Communications 13, 467, 2022). Breathing coordinates cortico-hippocampal dynamics in mice during offline states.

Kucharz et al. (Nature Communications, 2021). Post-capillary venules are the key locus for transcytosis-mediated brain delivery of therapeutic nanoparticles

Calia et al. (Nature Nanotechnology, 2021). Full-bandwidth electrophysiology of seizures and epileptiform activity enabled by flexible graphene microtransistor depth neural probes.

Dooley et al. (Current Biology, 2021). Movements during sleep reveal the developmental emergence of a cerebellar-dependent internal model in motor thalamus.

Lu, J., Tjia, M., Mullen, B. et al. (Mol Psychiatry, 2021). An analog of psychedelics restores functional neural circuits disrupted by unpredictable stress.

O. Ivashkina, A. Gruzdeva, M. Roshchina, K. Toropova, K. Anokhin (Int. J. Mol. Sci., 2021). Imaging of C-fos Activity in Neurons of the Mouse Parietal Association Cortex during Acquisition and Retrieval of Associative Fear Memory.

R. Padmashri, K. Tyner, A. Dunaevsky (J. Vis .Exp., 2021). Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice.

S. H. Bitzenhofer, J. A. Pöpplau, M. Chini, A. Marquardt, I. L. Hanganu-Opatz (Neuron,  2021). A transient developmental increase in prefrontal activity alters network maturation and causes cognitive dysfunction in adult mice.

Polina A. Egorova, Aleksandra V. Gavrilova, Ilya B. Bezprozvanny (Cell Calcium, 2021) In vivo analysis of the spontaneous firing of cerebellar Purkinje cells in awake transgenic mice that model spinocerebellar ataxia type 2.

W.Yang, M.Chini, J.A. Pöpplau, A.Formozov, A. Dieter, P. Piechocinski, C. Rais, F. Morellini,
O. Sporns, I. L. Hanganu-Opatz, J. S. Wiegert (PLoS Biology, 2021) Anesthetics fragment hippocampal network activity, alter spine dynamics, and affect memory consolidation.

E.Masvidal-Codina, T. M. Smith, D.Rathore, Y. Gao, X. Illa, E. Prats-Alfonso, E. Del Corro, A.Bonaccini Calia, G. Rius, I. Martin-Fernandez, C. Guger, P. Reitner, R. Villa, J. A. Garrido, A. Guimerà-Brunet, R.C. Wykes (Journal of Neural Engineering, 2021) Characterization of optogenetically-induced cortical spreading depression in awake mice using graphene micro-transistor arrays.

A.L.Keyesa, Y. Kimb, P. J.Bosch, Y.M.Usachev, G.M. Aldridge (Cell Calcium, 2021) Stay or Go? Neuronal activity in medial frontal cortex during a voluntary tactile preference task in head-fixed mice.

A. Bertolo, M. Nouhoum, S. Cazzanelli, J. Ferrier, JC. Mariani, A. Kliewer, B. Belliard, BF. Osmanski, T. Deffieux, S. Pezet, Z. Lenkei, M. Tanter (J. Vis. Exp, 2021) Whole-brain 3D activation and functional connectivity mapping in mice using transcranial functional ultrasound imaging.

M. A. Go, J. Rogers, G. Gava, C. Davey, s. Prado, Y. Liu, S. Schultz (Frontiers in Cellular Neuroscience, 2021) Place cells in head-fixed mice navigating a floating real-world environment.

A. Rakymzhan, Y. Li, P. Tang, R. K. Wang (Journal of Neuroscience Methods, 2021) Differences in cerebral blood vasculature and flow in awake and anesthetized mouse cortex revealed by quantitative optical coherence tomography angiography.

Lee HS, Han JH. (J Vis Exp., 2020) Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of a Freely Behaving Mouse

A.M. Ingiosi, C.R. Hayworth, D.O. Harvey et al. (Current Biology, 2020) A role for astroglial calcium in mammalian sleep and sleep regulation.

A.D. Umpierre, L.L Bystrom, Y. Ying, Y. U. Liu, G.Worrell, L.J. Wu (eLife, 2020) Microglial calcium signaling is attuned to neuronal activity in awake mice.

K. Juczewski, J. Koussa, A. J. Kesner, J. O. Lee, D. M. Lovinger (Scientific Reports, 2020) Stress and behavioral correlates in the head-fixed method: stress measurements, habituation dynamics, locomotion, and motor-skill learning in mice.

J. Ferrier, E. Tiran, T. Deffieux, M. Tanter, Z. Lenkei (PNAS, 2020) Functional imaging evidence for task-induced deactivation and disconnection of a major default mode network hub in the mouse brain.

Y. Li, A. Rakymzhan, P. Tang, R.K. Wang (Biomedical Optics Express, 2020) Procedure and protocols for optical imaging of cerebral blood flow and hemodynamics in awake mice.

A.D. Hogan-Cann, P. Lu, C. M. Anderson (PNAS, 2019) Endothelial NMDA receptors mediate activity-dependent brain hemodynamic responses in mice.

W. Sun, K. Suzuki, D. Toptunov, S. Stoyanov, M. Yuzaki, L. Khiroug, A. Dityatev (Frontiers in Neuroscience, 2019) In vivo Two-Photon Imaging of Anesthesia-Specific Alterations in Microglial Surveillance and Photodamage-Directed Motility in Mouse Cortex.

E. Pryazhnikov et al. (Scientific Reports, 2018) Longitudinal two-photon imaging in somatosensory cortex of behaving mice reveals dendritic spine formation enhancement by subchronic administration of low-dose ketamine.

H. Antila et al. (Scientific Reports, 2017)  Isoflurane produces antidepressant effects and induces TrkB signaling in rodents.

M. Kislin et al. (Journal of Visual Experiments, 2014)  Flat-floored air-lifted platform: a new method for combining behavior with microscopy or electrophysiology on awake freely moving rodents.


Piette, C. Hubert, A. Perez, S., Berry, H., Touboul, J.D., Venance, L. (2024). Striatal endocannabinoid-long-term potentiation mediates one-shot learning.

Stelzner, J.A., Krogsgaard, A., Kulkoviene, G., Sperling, L. & Lykke Lind, B. (2024). Astrocyte Contribution to Brain State-Dependent Neurovascular Coupling.

Chen, EL, Chen TW, Schreiter E.R., Lin BJ (2024). Synchronous ensembles of hippocampal CA1 pyramidal neurons associated with theta but not ripple oscillations during novel exploration.

Lee, D., Liu, L., Root, C. (2023) Transformation of valence signaling in a striatopallidal circuit.

Bosch, P. J., Kerr, G., Cole, R. C., Warwick, C. A., Wendt, L. H., Pradeep, A., Bagnall, E. (2023) Enhanced spine stability and survival lead to increases in dendritic spine density as an early response to local alpha-synuclein overexpression in mouse prefrontal cortex.

Lee, D., Liu, L. L., Root, C. M. (2023) Transformation of value signaling in a striatopallidal circuit

Umpierre, A. D., Li, B., Ayasoufi, K., Zhao, S., Xie, M., Thyen, G., Hur, B., Zheng, J., Liang, Y., Wu, Z. D., Yu, X., Sung, J., Johnson, A. J., Li, Y., Wu, L.-J. (2023). Microglial P2Y6 calcium signaling promotes phagocytosis and shapes neuroimmune responses in epileptogenesis.

Shi, L., Rodriguez-Contreras, A. (2022). The general anesthetic isoflurane inhibits calcium activity in cerebrovascular endothelial cells and disrupts vascular tone.

A. Virenque, R. Balin, F. M. Noe (2021) Dorsal skull meningeal lymphatic vessels drain blood-solutes after intracerebral hemorrhage.

Video articles in the Journal of Visualized Experiments (Jove)

Bertolo, A., et al. (2021) Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging.

Kislin, M., et al. (2014) Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents.

How to cite us

We would appreciate you mentioning our equipment when you publish: please refer to our equipment as the Mobile HomeCage (or HeadFix, if you are using the head fixation part only) and to our company as Neurotar. Thank you!

Educational webinars

Vincent Magloire, a Research Fellow at the University College London, and Ken Berglund, an Assistant Professor at Emory University (USA), will present their work on the spatiotemporal dynamics of neurotransmitter and calcium imaging during seizure evolution in awake head-fixed mice and models of epilepsy. Click on the image below and register to attend on March 21, 2024, or receive the recording.

Alexey Semyanov, Director of the Neuroscience Center at Jiaxing University (China), and Amit Agarwal, PI at the University of Heidelberg (Germany) will show you the astrocytes and the oligodendrocyte precursor cells (OPCs) as you have not seen them before. Learn about their interactions with neurons and responses to calcium signaling during locomotion. Click on the image below and register to attend the live webinar on December 14th, 2023, or receive the recording.

Developing Prefrontal Circuits and Their Role in Health and Disease. An introduction by Ileana Hanganu-Opatz, PI, Director of the Institute for Developmental Neurophysiology at the University Medical Center Hamburg-Eppendorf followed by presentations by two lab members, Jastyn A. Pöpplau, and Marilena Hnida. Click on the image below and watch the webinar on demand. February 16, 2023.

Neurovascular Coupling: Novel Insights from Studies in Awake Head-Fixed and Anesthetized Mice. Presenters: Barbara Lind (Assistant Professor at the University of Copenhagen) and Adrián Rodríguez-Contreras (Associate Professor at Northwestern University). November 3, 2022. Click on the image below to watch the webinar on demand. March 11, 2022.

Sensorimotor Network Development During Early Postnatal Life in the Awake and Sleeping Brain. Presenters: Anna Cavaccini (Theofanis Karayannis lab, University of Zurich), James Dooley (Mark Blumberg lab, University of Iowa). September 2, 2021.

Hippocampal Neural Circuits in Awake Head-Fixed Mice Navigating a Real-World Environment. Presenting labs: Simon Schultz (Imperial College London), Michael Goard (University of California at Santa Barbara). February 18, 2021.

Studying Epilepsy in Awake Head-Fixed Mice Using Microscopy, Electrophysiology, and Optogenetics. Presenting labs: Longjun Wu lab (Mayo Clinic, USA), Rob Wykes lab (UCL, UK). October 8, 2020

Functional Ultrasound (fUS) Imaging in the Brain of Awake Behaving Mice. June 30, 2020.

Place Cell Mapping and Stress Monitoring in Head-Fixed Mice Navigating an Air-Lifted Homecage. August 23, 2019

Single-Cell Electrophysiology and 2-Photon Imaging in Awake Mice with 2D-Locomotion Tracking. February 6, 2019

Making Optical and Electrophysiological Measurements in the Brain of Head-Fixed, Freely-Moving Rodents. March 25, 2015


User labs

Aarhus University - user lab - Neurotar
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