Want to find out whether the Mobile HomeCage fits into your experimental set-up? In this guide to compatibility, we provide the instructions for taking the microscope measurements, selecting a stage, and an air source. If you don’t want to measure the microscope yourself, tell us the maker and the model. Ideally, also send us a front and side picture of your setup with a ruler.
If you are measuring the microscope yourself, watch the video below and read the instructions on taking the measurements.
The Mobile HomeCage models
Let’s recapitulate some basic facts about the Mobile HomeCage. It comes in two sizes: the standard size Mobile HomeCage is compact and compatible with the majority of commercial 2-photon microscopes. The Mobile HomeCage Large allows combining imaging with behavioral readouts. For this reason, the floating cage has a larger diameter (325 mm compared to 180 mm in the standard-size Mobile HomeCage). The frame is also larger: it requires more space in the imaging setup. Both systems can be equipped with locomotion tracking components. Luckily, the sensor board used for tracking is built into the air dispenser. It does not require additional space in the setup. Whether you are using the system with or without locomotion tracking, it takes up the same space.
Measuring the microscope for compatibility
The picture on the left is a symbolic representation of a microscope frame. Letters A through F stand for critical dimensions. Pay special attention to the first three measurements.
The table below explains what the critical dimensions are, what their values must be, and what to do if your measurements do not match the given values. The values are given for the default configuration with the standard rotating clamp:
|A. Distance from the objective's focal |
point to the microscope frame must be
|>122 mm||>267 mm||Mobile HomeCage: use an elongated clamp (min distance with elongated clamp 102 mm);
Mobile HomeCage Large: use a smaller cage diameter (ask us about customization)
|B. Distance from the objective's focal point (objective retracted) to the table / stage surface must be||>70 mm||>84 mm||Lower the stage (to increase the distance); lift the stage or use a riser block (to decrease the distance)|
|C. Objective's parfocal length must be||>40 mm||>40 mm||Use a parfocal length extender to increase the objective's length or use a low-wall cage with a short neck|
|D. Objective's length with holder / slider must be||>56 mm||>56 mm||Use a short neck and a low-wall cage|
|E. Objective's diameter||Consider alongside WD||Consider alongside WD||The maximum objective's diameter depends on the working distance (WD). Up to 100 mm diameter objectives can be compatible under certain conditions - contact us for help|
|F. Objective's holder diameter must be||<80 mm||<80 mm||Use an elongated clamp|
The Mobile HomeCage® Large is compatible with these commercial two-photon microscopes:
- without modifications: Scientifica’s Hyperscope (VivoScope frame), Neurolabware, Nikon A1R (gantry frame), Sutter MOM, LaVision Biotech’s TriM Scope Matrix, Femtonics’ FemtoS-Bridge and Olympus FVMPE-RS (with InnerFocus);
- with some cage modifications: Bruker’s Ultima Investigator and 2P Plus models, Thorlabs’ Bergamo II and DIY 2-photon;
- Finally, it is possible to use the Mobile HomeCage Large system with most commercial microscopes by adding a periscopic extension, such as the Inverterscope available from LSM Tech.
Selecting a stage
Make sure that the stage can carry the weight of your Mobile HomeCage device. The stage surface can be slightly smaller than the size of the air dispenser. Check the table below for the unpackaged weights and air dispenser sizes (length X width) of the Mobile HomeCage systems:
|Type of device||Unpackaged weight (kg)||Air dispenser dimensions (mm)|
|Mobile HomeCage||6.1||430 X 300|
|Mobile HomeCage with tracking capability||6.8||430 X 300|
|Mobile HomeCage Large||11.5||720 X 570|
|Mobile HomeCage Large with tracking capability||13||720 X 570|
|MultiCage Training Arena||11.5||720 X 570|
Selecting an air source
Finally, you would need a high flow – low-pressure air source to operate your Mobile HomeCage device. Most labs are able to use their local laboratory air sources, but some require commercial air pumps. Any pump operating at or above the specifications (below) would work. We recommend using industrial air pumps, although many cost-efficient aquarium pumps work quite well and some are available on Amazon. Unfortunately, most aquarium pumps don’t allow adjusting the airflow. If this is the case, we recommend purchasing an airflow regulator. Contact us for specific air pump recommendations. Before you do, check whether your locally available air source satisfies these requirements:
|Type of device||Air source specifications|
|Mobile HomeCage||120 L/min at 20 kPa|
|Mobile HomeCage with tracking capability||120 L/min at 20 kPa|
|Mobile HomeCage Large||150 L/min at 20 kPa|
|Mobile HomeCage Large with tracking capability||150 L/min at 20 kPa|
|MultiCage Training Arena||250 L/min at 20 kPa|
We do not recommend using compressed air tanks as an air source for the Mobile HomeCage: due to high pressure, they run our of air very quickly.
We are still working on this page and we shall be adding more information soon. If you don’t find the information that you need, contact us for help.